Affymetrix | Panomics Solutions
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ViewRNATM CTC Platform to isolate and characterize circulating tumor cells (CTCs)

CTCs isolation and characterization workflow

Blood Sample: Blood sample is collected in a K2-EDTA tube.

Isolated CTCs: Blood is diluted in specific buffer and filtered through isolation device in less than 3 minutes. The bottom portion of device is removed and the capture filter is released into a 24-well plate for ViewRNA Assay.

Characterize CTCs: Sample is prepared (fixed, baked, permeabilized and protease digested) to enable RNA accessibility. Target RNA Probe Sets are hybridized followed by a sequential hybridization of signal amplification and detection components. Once processed, filters are transferred to a microscope slide for image processing and analysis.