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ViewRNATM CTC Platform to isolate and characterize circulating tumor cells (CTCs)

Assay Performance Highlights

ScreenCell® filtration device - isolation of circulating tumor cells
Sample Type Peripheral blood or PBMC
Species Human
Isolation Type Live cells Fixed cells
Cell Culture expansion Yes No
Max. Blood input vol. 6 mL 3 mL
ViewRNA Assay - characterization
Plex level 1 to 4-plex
Assay format 24-well plates
Sensitivity 1 RNA molecule
Detection Flourescence
Instrumentation Flourescence microscope or imaging system


Sensitive isolation of CTCs using ScreenCell Cyto isolation device

CTC isolation using ScreenCell Cyto

The ScreenCell Cyto device is able to isolate fixed tumor cells with a high recovery rate (Table 1). Cells are well preserved morphologically, and the ViewRNA Assay can be performed directly on the filter without additional processing steps. Read More ...

 


RNA in situ hybridization assay specificity

RNA in situ  analysis CTC FISH

Human PC9 lung carcinoma cells were used to determine the ViewRNA Assay specificity. PC9 cells were labeled with CellTracker® Green and were counted before and after the assay. In addition, PC9 cells were probed for KRT19 mRNA, and the number of KRT19 positive cells was compared with the number of CellTracker Green positive cells. The assay was highly specific in detecting green labeled PC9 cells (representing CTCs) expressing the KRT19 biomarker with an average specificity of 97% (Figure 1, Table 2). Read More ...

 


Detection of HER2 in human breast carcinoma SK-BR-3 cells

tissue microarray (TMA) RNA in situ  analysis in CTCs FISH

HER2 gene is known to be amplified in human breast SK-BR-3 cells. SK-BR-3 cells were spiked in 3 mL human whole blood and captured by the ScreenCell filter, followed by ViewRNA Assay using probes specific to HER2 (green), KRT19 (red), and CD45 (magenta). The results showed a high number of dots (puncta) per cell for HER2 and KRT19, demonstrating high RNA expression for both genes in SK-BR-3 cells. CD45 is not expressed in SK-BR-3 cells (negative control) and was not detected in this experiment. Read More ...

Selected Publications

ViewRNA Complete Bibliography Click here