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Fluorescence RNA In Situ Hybridization (RNA FISH)—Overview

RNA_in_situ_hybridization_FISH_HC_high_content_screening

Fluorescence based in situ hybridization (FISH) techniques are used to visualize DNA or localized RNAs within cells. However the fluorescence in situ hybridization of RNA has always been limited by low sensitivity, complicated workflow and the inability to perform multiplex analysis. ViewRNA HC Screening Assay is a novel non-radioactive RNA in situ hybridization solution that offers single-molecule RNA sensitivity and the ability to multiplex up to 4 RNA targets in adherent or suspension cells. The assay is optimized for automation and can be performed in 96- or 384-well plates.

Unique Benefits

  • Directly visualize RNA in adherent or suspension cells
  • Sensitivity of one copy/cell at single cell resolution
  • Analyze one to four mRNA targets per cell
  • High-throughput and high-content compatible
  • Compatible with standard fluorescence microscopes or high content imagers

Applications

  • siRNA knockdown studies
  • Stem cells and developmental biology
  • Biomarker validation
  • Analysis of transcriptional heterogeneity
  • Reporter gene assays
  • Time course studies
  • Viral gene trafficking
  • A companion to immunofluorescence (IF) or DNA FISH assays

Assay Performance Highlights

ViewRNATM HC Screening Assay

Sample Type Cultured cells (adherent and suspension)
Species Any
Limit of Detection (LOD) 1 mRNA molecule/cell
Plex Level 1 to 4-plex
Assay Format 96- or 384-well plates
Detection Fluorescent
Instrumentation Imaging system of epi-fluorescence microscope