Affymetrix | Panomics Solutions
+1.877.726.6642
Quantitative Biology Delivered.

ViewRNA® ISH Tissue Assay for RNA In Situ Hybridization (RNA CISH/RNA FISH)

An incredibly precise assay.


ViewRNA_manual_assays_how_it_works

 

WISH RNA ISH assays were

easier to do?

ViewRNA Assays components are developed to work together to achieve the highest possible signal, lowest background, and most stringent specificity.
  • Unhindered by crosslinking
  • Probes readily hybridize, even to partically degraded RNA sequences
Assay_Components_Automation

 

RNA_in_situ_hybridization-probe_design

WISH you had a better RNA

ISH probe? 

Leveraging over 23 years of RNA bioinformatics expertise, Affymetrix is the leader in designing and manufacturing the most specific and sensitive assays for RNA in situ visualization. Each target RNA sequence is identified using synthetic probes consisting of as many as 40 oligonucleotides-target specific oligonucleotides and "blocking oligonucleotides" designed to reduce non-specific binding. Each oligonucleotide has two components: a lower region that is complementary to the target RNA and an upper region that provides the binding site for the branched-DNA signal amplification structure.

Our assays

 

Ultra-sensitive signal.

Up to 8,000 fold amplification.

Advancements in branched-DNA signal amplification technology allow for single molecule detection of the target RNA. The amplification structure is built onto the upper region of the probes through sequential hybridization of a series of amplifiers and label probes. The chromogen(s) form a precipitate wherever the target RNA molecules are located. Each signal dot corresponds to one target molecule. To quantify target expression, simply count the dots.
Signal_amplification-branched_DNA_sensitive.jpg

 

RESULTS

IN HOURS


NOT DAYS

Fast results. Fast answers. 

The ViewRNA Assays allow you to perform RNA in situ hybridization assays with unprecedented speed. Now you can run the assay and have results in just one day, not days or weeks. Which means a fast response to support a faster, better, more informed decision.

The entire workflow can be finished in 7 hours (1-Plex) to 9 hours (2-Plex).

 

Better results.

Better decisions.

By using ViewRNA Assays you will gain a better understanding of the disease, enabling you to make better decision. With single RNA molecule sensitivity and essentially no background, you can trust that the signal reveals the biology and is not due to a staining artifact. At Affymetrix we are experts at assays development and when using our assays you can have the confidence that the results are revealing the biology in the sample.

For the first time to you take your science to the next level by visualizing novel targets (like circular RNA), or targets for which an antibody is not readily available.
ViewRNA_circular_non-coding-RNA_lncRNA
CircHomer1_a (green) in mouse hippocampal slices, visualized using ViewRNA Assays, and Map2 (antibody, red).

 

Any_species_any_target_RNA_in_situ_hybridization

There is a ViewRNA Assay

for everyone.

ViewRNA Assays makes any sample type you have easy to analyze. Struggling for weeks and months to find an antibody towards dolphin, monkey, plant or chicken? No problem - with ViewRNA you can visualize your target of interest in any species.

Trying to make IHC work in FFPE samples, tissue microarrays (TMAs) or fine needle aspirates (FNAs)? ViewRNA delivers robust results in any sample type!