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ViewRNATM miRNA ISH Cell Assay for Fluorescence miRNA and RNA In Situ Hybridization (RNA FISH)

ViewRNA miRNA ISH Cell Assay:

Summary of all of the kits, materials, and equipment necessary to run a ViewRNA miRNA ISH Cell Assay. PDF

Required components, each sold separatelly:

  • ViewRNA miRNA ISH Cell Assay Kit—contains all the assay reagents for signal amplification and detection required for visualization of 1 target miRNA and 1 target RNA using adherent or suspension cells.
  • ViewRNA miRNA Probe Set—miRNA target specific probes (TYPE 1)
  • ViewRNA Probe Set—RNA (mRNA or lncRNA) target specific probes (TYPE 4)

Optional kits, each sold separatelly:

  • ViewRNA ISH Cell 740 Module—designed to be used in conjunction with ViewRNA miRNA ISH Cell Assay and allows analysis of an additional RNA target in the 740 channel. PDF
  • ViewRNA TYPE 10 Probe Set—RNA target probe set for viewing in the 740 channel. See the Probe Set Catalog.
ViewRNA ISH Cell 740 ModuleKitQVC0200
ViewRNA miRNA ISH Cell Assay Kit24 assays/144 assays*QVCM0001

* 24 assays when using cover slips processed in 24-well plate
  144 assays when processing samples in an optical bottom 96-well plate


ViewRNA miRNA TYPE 1 Probe Sets:

ViewRNA miRNA TYPE 1 Probe Sets are designed, manufactured and packaged for use with the ViewRNA miRNA products. TYPE 1 Probe sets desinged using fast red substrate. For a complete list of all available ViewRNA miRNA Probe Sets see the Probe Set Catalog. Read More

ViewRNA miRNA TYPE 1 Probe Set, By Request44 uLaVM1-99999-01
ViewRNA miRNA TYPE 1 Probe Set, By Request1.44 mLbVM1-99999-02
ViewRNA miRNA TYPE 1 Probe Set, By Request7.2 mLcVM1-99999-03

a Sufficient for 110 assays
b Sufficient for 360 assays
c Sufficient for 1800 assays


ViewRNA TYPE 4 & TYPE 10 Probe Sets:

ViewRNA TYPE 4 and 10 Probe Sets are designed, manufactured and packaged for use with the ViewRNA products. ViewRNA TYPE 10 Probe Sets—(Optional) RNA target probe set for viewing in the 740 channel. For a complete list of all available ViewRNA TYPE 4 Probe Sets see the Probe Set Catalog. Read More

ViewRNA TYPE 4 Probe Set0.44 mLaVX4-99999-01
ViewRNA TYPE 10 Probe Set0.44 mLaVX10-99999-01
ViewRNA TYPE 4 Probe Set1.44 mLbVX4-99999-02
ViewRNA TYPE 4 Probe Set7.2 mLcVX4-99999-03
ViewRNA TYPE 10 Probe Set1.44 mLbVX10-99999-02
ViewRNA TYPE 10 Probe Set7.2 mLcVX10-99999-03

a Sufficient for 110 assays
b Sufficient for 360 assays
c Sufficient for 1800 assays


ViewRNA miRNA Accessory Items:

Additional materials and components, not supplied in the reagent kit, that may be required but not provided, based on assay format.

Forceps*eachQVC0501
Tissue Culture 24-well Plate*eachQVC0504
Microscope Slides*12 eachQVC0505
Cover Slips*50 eachQVC0507

* Part of ViewRNA ISH Cell Accessory Kit


ViewRNA miRNA ISH Cell Individual Components:

Additional components sold seperately.

10X PBS42 mLQVC0508
Detergent Solution QC11 mLQVC0509
Protease QS22 uLQVC0511
Probe Set Diluent QF12 mLQVC0512
Label Probe Diluent QF12 mLQVC0514
100X DAPI105 uLQVC0515
Fast Red Substrate (41 mL Naphthol Buffer + 8 Fast Red Tablets)96 assaysQVT0505
AP Enhancer Solution10 mLQVT0508
AP Enhancer Solution40 mLQVT0509
View miRNA ISH Cell Wash Buffer Components 1 + 224 assays/144 assaysQVCM0500
View miRNA ISH Cell Cross-Linking Buffer QM40 mLQVCM0501

ViewRNA Temperature Validation Kit:

The View Temperature Validation Kit uses a calibrated digital thermometer to assess temperature accuracy. This kit may be used for any ViewRNA Assay to evaluate the temperature of incubators or ThermoBrite systems.

View Temperature Validation KiteachQV0523

 


QuantiGene Incubator Temperature Validation Kit:

The QuantiGene Incubator Temperature Validation Kit uses a calibrated thermometer to assess the accuracy of the temperature of the oven incubator or shaking incubator used in all QuantiGene Assays.

QuantiGene Incubator Temperature Validation Kit1 kitQS0517

Horizontal Air Flow Ovens from Affymetrix:

  • Recommended oven for QuantiGene assays
  • Provides optimal temperature uniformity and recovery without overshoot
  • Easy-to-read LED displays temperature
  • Stainless steel interior and shelves
  • Three Sizes - 1.6, 3.0 or 5.0 cu. ft., 110V or 220V
  • CE Approved

Horizontal Air Flow - How it Works Read More ...

Specifications Read More ...

Horizontal Air Flow Oven, 120V1.6 cu. ft.QS0704
Horizontal Air Flow Oven, 120V3 cu. ft.QS0700
Horizontal Air Flow Oven, 120V5 cu. ft.QS0701
Horizontal Air Flow Oven, 220V1.6 cu. ft.QS0712
Horizontal Air Flow Oven, 220V3 cu. ft.QS0710
Horizontal Air Flow Oven, 220V5 cu. ft.QS0711
Extra Shelf, 3 cu. ft. oveneachQS0702
Extra Shelf, 5 cu. ft. oveneachQS0703